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KMID : 1149220210290040181
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Korean Journal of Oriental Medical Prescription
2021 Volume.29 No. 4 p.181 ~ p.189
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Protective Effects of the Calyx of Diospyros kaki Thunb. on H2O2-induced Oxidative Stress in C2C12 Myoblasts
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Park Jeong-Hwan
Lim Jin-Yeong
Lee MIn-A
Seong Eun-Jeong
Lee Jun-Soo
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Abstract
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Objectives : The calyx of Diospyros kaki Thunb. has usually been used to treat obstinate hiccup. This study investigated the protective effects of Diospyros kaki using C2C12 myoblasts of H2O2-induced oxidative stress.
Methods : Cell viability and cytotoxicity were determined by MTT assay. The level of reactive oxygen species (ROS), malondialdehyde (MDA), and reduced glutathione (GSH) were measured by using optical properties.
Results : The calyx of Diospyros kaki Thunb. extract showed no toxicity to C2C12 myoblasts until 20 ㎍/mL concentration and increased cell viability compared to the H2O2 treated group. The calyx of Diospyros kaki Thunb. extract inhibited the production of ROS and MDA at all concentrations. In addition, the calyx of Diospyros kaki Thunb. extract increased the concentration of GSH.
Conclusion : This study provides that the calyx of Diospyros kaki Thunb. can be used as a potential material that exhibit antioxidative and protective effects on H2O2-induced oxidative stress in C2C12 Myoblasts.
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KEYWORD
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Diospyros kaki Thunb., C2C12 myoblasts, antioxidant activity, oxidative stress, reactive oxygen species
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